#PUC57 SNAPGENE HOW TO#
#PUC57 SNAPGENE DRIVERS#
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Among several health beneficial effects, the anticancer activity of LAB remains the most interesting and controversial property. LAB are nonpathogenic, Gram-positive and generally recognized as safe bacteria. Probiotics, such as lactic acid bacteria (LAB), have recently gained considerable importance because of their health beneficial properties, such as immunomodulatory, anti-inflammatory and anticarcinogenic properties (Zhu et al. In this context, a new promising area is utilizing biological agents like probiotics. Despite considerable progress in the treatment of colorectal cancer in the last two decades, there is still a great need for the development of therapeutic methods. The use of recombinant probiotics that produce anticancer compounds is a promising option for combating cancer cells.Ĭolorectal cancer is the third most common cancer and the fourth cause of cancer-related deaths worldwide (Ferlay et al. lactis producing TRAIL protein can induce apoptosis in human colon adenocarcinoma cell lines SW480 and HCT116. A significant change was observed in the ratio of Bax/Bcl2 expression in HCT116 cells only following treatment with the supernatant of recombinant L. The viability of SW480 and HCT116 cells treated with recombinant L. Gene expression of apoptosis genes, Bax and Bcl2, was assessed by qPCR. Induction of cell death was evaluated by coculturing transformants producing either form of TRAIL protein with the two cell lines followed by MTT assay. lactis was confirmed by Western blotting and immunofluorescence. Methods and ResultsĬonstructs were designed to produce either secreted or cell wall-anchored forms of human TRAIL cloned into pNZ7021 expression vector. The expressed protein was either cell wall anchored or secreted, and it was assessed whether this could induce apoptosis in human colon adenocarcinoma cell lines SW480 and HCT116. 4.We investigated the ability of Lactococcus lactis, a species generally regarded as safe, to express tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) protein. Questions : If you have any questions, please contact our Scientific Support Team at 0 ext. They also offer maxi-prep of plasmids, with amounts up to 30 mg. Therefore, in order to receive a price quote, work order, and ETA specific for your project, please send all your project details to Services: Additional services offered by Bio Basic include codon optimization, for no additional charge.

#PUC57 SNAPGENE HOW TO#

How to Receive a Price Quote: Bio Basic reviews all sequences before accepting a project.

This can be supplied in any format, including WORD doc or SnapGene file.

Final Expected Sequences of Plasmid(s), and relevant cloning instructions for BioBasic to follow (such as cloning sites to be used).

When sending us instructions for your subcloning project, please provide:

#PUC57 SNAPGENE DRIVERS#

Our drivers take care of picking up the materials from your lab and shipping them to BioBasic for a fee of $40. Subcloning into one of the following vectors costs a standard subcloning price of $150:Ĭustom Vector Subcloning: Lastly, you can send your own vector to BioBasic, and they will perform the cloning for you (for the same fee of $150).

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Subcloning into one of the following vectors is also free of charge:īio Basic keeps many vectors in-house, shown below. Subcloning Services: The default is blunt end cloning into pUC57 with either Amp or Kan resistance.

All genes are verified by Sanger Sequencing, as well as other QC measures. What do I receive? Bio Basic supplies 4 μg of lyophilized plasmid containing your gene, and up to 10 μg for no extra charge.

The proposed standard TAT gene synthesis is based on a gene with little to no complexities and with a length of about 1,000 bases. *Please note that the turnaround time is dependent on the complexity and length of a gene. Custom Genes:įrom the easiest to the most complicated gene syntheses, Bio Basic has the expertise. Our staff of experienced molecular biologists will be happy to assist you. For technical assistance contact us at call 0 ext. We will send you a price quote and, upon your approval, procure and deliver your peptide. All peptides quality confirmed by Mass Spec and HPLCįill out this form to send us the details of the custom peptide you want.Custom Peptides & Genes from Canada-based Bio-Basic Inc.

Puc57 snapgene